AP2A1 Knockout cell line (Hep G2)

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AP2A1 Knockout cell line (Hep G2) Order

AP2A1 Knockout cell line (Hep G2)
Catalog#
YKO-HT6239
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AP2A1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AP2A1 Knockout cell line (Hep G2)
Species
Human
Gene Name
AP2A1
Gene ID
160
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript AP2A1-201)
Validation: PCR+Sanger Sequencing
AP2A1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AP2A1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AP2A1 gene knockout AP2A1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AP2A1 Knockout cell line (Hep G2)
AP2A1 Knockout cell line (Hep G2)
Catalog#
YKO-HT6239
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AP2A1 Knockout cell line (HeLa)

    AP2A1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-H1191
    YKO-H1191(Hela)
  • AP2A1 Knockout cell line (HCT 116)

    AP2A1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20671
    YKO-XN20671(HCT 116)
  • AP2A1 Knockout cell line (HEK293)

    AP2A1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20670
    YKO-XN20670(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • AP2A1 Knockout cell line (HeLa)

    AP2A1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-H1191
      inquiry   More details
  • AP2A1 Knockout cell line (HCT 116)

    AP2A1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20671
      inquiry   More details
  • AP2A1 Knockout cell line (HEK293)

    AP2A1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20670
      inquiry   More details

AP2A1 Knockout cell line (Hep G2) Order

AP2A1 Knockout cell line (Hep G2)
Catalog#
YKO-HT6239
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AP2A1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AP2A1 Knockout cell line (Hep G2)
Species
Human
Gene Name
AP2A1
Gene ID
160
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript AP2A1-201)
Validation: PCR+Sanger Sequencing
AP2A1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AP2A1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AP2A1 gene knockout AP2A1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AP2A1 Knockout cell line (Hep G2)
AP2A1 Knockout cell line (Hep G2)
Catalog#
YKO-HT6239
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AP2A1 Knockout cell line (HeLa)

    AP2A1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-H1191
    YKO-H1191(Hela)
  • AP2A1 Knockout cell line (HCT 116)

    AP2A1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20671
    YKO-XN20671(HCT 116)
  • AP2A1 Knockout cell line (HEK293)

    AP2A1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20670
    YKO-XN20670(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • AP2A1 Knockout cell line (HeLa)

    AP2A1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-H1191
      inquiry   More details
  • AP2A1 Knockout cell line (HCT 116)

    AP2A1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20671
      inquiry   More details
  • AP2A1 Knockout cell line (HEK293)

    AP2A1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: AP2A1
    Gene ID: 160
    Size: 1*10^6
    Catalog#: YKO-XN20670
      inquiry   More details

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